Eliciting Dual-Niche Immunological Priming by Acupoint Delivery of Nanovaccines

• Acupoint delivery of nanovaccines (ADN) simultaneously stimulates mast cell-assisted dendritic cell maturation at the acupoint and directly enriches nanovaccines into draining lymph nodes, achieving dual-niche immunological priming. • ADN enhances antigen presentation by lymph node-resident CD8α+ dendritic cells and induces nanovaccine accumulation in B-cell zones, amplifying cytotoxic T lymphocyte responses and IgG antibody expression. • ADN generates systemic immune responses by inducing immune memory and preventing T-cell anergy in the spleen, leading to effective antitumor and antiviral immunity in mice. • This facile and versatile platform offers a promising strategy for advanced nanovaccination, potentially improving vaccine efficacy against tumors and infectious diseases.