• Development of a novel center-concave core-shell WO3@Prussian blue nanosheet architecture that synergistically enhances enzymatic oxidation and H2O2 reduction for ultrasensitive lysine detection.
• Integration of the nanocomposite into a handheld microchip-based device enabling rapid (10 s) and precise lysine quantification in blood, suitable for point-of-care and home monitoring.
• The device overcomes limitations of traditional methods (cost, time, complexity) offering a portable, plug-and-play solution for clinical and nutritional applications.
• Demonstrated outstanding accuracy in blood analysis, highlighting potential for early disease diagnosis and dietary management.