• Developed a dual-enzyme cascade system integrating iridium single-atom nanozymes with glucose oxidase, optimizing hydrogen peroxide production, acidity, and temperature within the tumor microenvironment to markedly improve catalytic therapy efficacy.
• Functionalized the cascade reaction system with cancer cell membranes to achieve homologous targeting and high biocompatibility, ensuring accurate accumulation at tumor sites while minimizing off-target effects.
• Demonstrated that GOx catalysis produces abundant H2O2 and lowers local pH, while laser irradiation enables mild-temperature photothermal enhancement, collectively maximizing the catalytic efficiency of Ir SAzymes.
• Presented a comprehensive strategy for refining single-atom catalytic kinetics, offering a promising approach for tumor homologous-targeted catalytic therapy with potential clinical translation.