• Scanning strategy significantly influences the anisotropy of microstructure and mechanical properties in LPBF-formed GH3536 alloy.
• The 67° scanning strategy yields the best combined tensile properties at room temperature and 815 °C, while 0° and contour strategies result in the worst.
• Anisotropy is attributed to differences in texture strengthening, grain boundary strengthening, and dislocation strengthening effects.
• Modulating the scanning strategy can effectively mitigate mechanical property anisotropy in LPBF-formed GH3536 alloy.