• The as-prepared sensors can detect NH4+ in body fluids with a high sensitivity of 58.7 mV decade−1 and an ultrawide detection range of 1–100 mM.
• The biocompatible sensors exhibit desirable biocompatibility and minimal toxicity for continuous and long-term monitoring.
• The average detection error of the integrated and wireless biosensing patch was 13.2%, and body fluid detection accuracy is improved by more than 18% after cross-calibration.
• The sensor array with cross-calibration effectively eliminates potassium ion interference, achieving a selectivity coefficient of 0.11.
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